Confocal Raman Imaging Microscopy Systems
Confocal Raman Imaging Microscopy
NRS-4500 Confocal Raman Imaging Microscope with High Speed QRi
The NRS Series for confocal Raman imaging microscopy includes three different optical configurations; the easy to use, yet powerful NRS-4500, with up to three laser wavelengths (from a wide range of visible to NIR) including the common dual laser 532/785nm configuration). The sophisticated NRS-5000 Series, with 300mm spectrograph, offering higher spectral and spatial resolution with the use of dual spatial filtration (DSF) and the NRS-7000 Series with 500mm spectrograph and poly-dispersive options. Both the NRS-5000 and NRS-7000 can be configured with up to 8 lasers (9 wavelengths). All models include Class 1 laser safety as standard and share the same comprehensive Spectra Manager™ Suite of measurement and micro-imaging software.
System Features
High accuracy spectral resolution, with the use of rotary encoders for the grating drive mechanism to as little as 0.3cm-1 per pixel
High spatial resolution with 0.1µm step, using the automated XYX stage (actual resolution depends on laser wavelength and objective magnification and N.A.)
QRi (Quick Raman imaging) – a combination of EMCCD with an ultra-fast XYZ stage giving 0.1µm step resolution QRI increases the data acquisition speed by up to 50x compared with a conventional mapping stage and also offers a significant improvement in sensitivity. Available on all models
SPRinTS (Software Programmable Raman Integration Speed) which uses Vertiscan to scan the laser through the objective to map samples that are likely to move using a conventional mapping stage, such as liquids and suspensions. Available on the NRS-5000 and NRS-7000 models only
Long working distance stage and objectives, for larger samples and temperature control and high pressure accessories
Optional fiber probe interface for external measurements
Macro objective for measurement of powders and liquids
Patented Fluorescence Rejection, an algorithm to reduce or eliminate fluorescence an enhance Raman measurement. Available on all models
Spectra Manager™ Imaging Suite with KnowItAll®, for comprehensive measurement, analysis and reporting. The cross platform software includes the same feature for all NRS models with many options for chemometrics, spectral analysis, etc.
Introduction to the NRS-4500 Confocal Raman Microscope
NRS-4500 Confocal Raman Microscope
NRS-4500 Confocal Raman Microscope
The NRS-4500 Series Confocal Raman Microscope is a purpose designed Raman micro-spectrophotometer, with a dedicated f200 spectrograph including up to 4 gratings that can be selected automatically and detection by high performance Andor CCDs (with options for electron multiplying EMCCDs). The optical system includes Olympus optics with a choice of objectives from x5 to x100 with options for NIR and long working distance for working with sample stages. High clarity imaging is ensured with a super resolution CMOS camera.
Class 1 Laser Safety
The standard Class 1 safety cabinet more commonly associated with high-end Raman systems.
Confocal Imaging
An automatic XYZ stage is used for confocal imaging with X/Y spatial resolution to 1 µm (Z=1.5 µm). Spectra Manager™ Suite with the Imaging Analysis application is used to collect a three dimensional image of the sample which can be rotated and viewed from any angle. The image map is created using peak data corresponding to key functional groups and analysis of multiple peaks can be combined to create powerful data images.
Rigid Optical Bench and Laser Image
The microscope has a completely rigid structure to prevent flexing and is not built around an optical microscope.
Switching between observation and measurement modes is completely automatic and can be done with the safety cabinet closed.
Standard Configuration
532/785nm Lasers with Matching Edge Filters
NRS-4100 Dispersive Confocal Raman MicroscopeThe NRS-4500 offers several standard configurations including the typical 633nm single laser or 532/785nm laser combination with matching notch or edge filters, with an option for a third laser which can be mounted internally or externally. Observation of the laser spot ensures perfect alignment of the target sample position and provides an indication of surface roughness. All laser wavelengths are selected in the software and once selected, the optical system (including the laser) is automatically aligned for optimal throughput and resolution. Four software selectable gratings control the spectral range and resolution from 8000 to 100 cm-1 (8000 to 50 cm-1 as an option). The grating direct drive system includes a rotary encoder to ensure excellent wavelength reproducibility ±0.2cm-1.
Fluorescence Rejection
457nm Laser and Patented Fluorescence Rejection Algorithm
NRS-4100 Dispersive Confocal Raman MicroscopeJASCO has developed new and novel (patented) mechanisms to deal with sample fluorescence. As with other Raman systems, we can utilize laser wavelengths of 785 nm and up to 1064 nm, but we have recently incorporated a 457 nm laser that offers significantly higher Raman signal, improved spatial resolution and much lower fluorescence for many different sample types. Selecting a different excitation laser wavelength is only one of the ways the NRS-4500 minimizes fluorescence interference. The Fluorescence Rejection algorithm (patented) included in the Spectra Manager™ can effectively remove or minimize fluorescence regardless of the laser wavelength used either at the time of measurement or during post-processing.
Flexible Sampling Options
With a choice of refractive objectives, both micro and macro measurements are possible as well as options for long working distance objectives for heating/cooling stages and other sampling accessories. The NRS-4500 can be used with either a manual sample stage or the PC-controlled, automated XYZ confocal mapping stage. A fiber probe can be added for external measurement.
Powerful ‘UserAssist’ Control for Experienced Spectroscopists and New Users
The ‘UserAssist’ software aids the user in setting up the NRS-4500 for sample measurement; a simple sequence guide takes you through setup and optimization of measurement parameters with helpful advice and tips, such as a warning if you have the laser intensity set too high. When each of the parameters has been set, the NRS-4500 automatically selects the laser and matching notch filter, the grating for the appropriate resolution, focuses on the sample and then the spectral measurement is performed.
‘Simple Search’ Function
The new ‘Simple Search’ function is used with the automated XYZ stage. A new algorithm developed by JASCO (patent pending) analyzes the microscopic image and automatically selects measurement position(s) based on size, contrast and/or color of the target material. The user simply clicks the measurement button to execute spectral measurements of the desired sample positions.
Chemical Image Identification and Functional Groups Registry
To provide faster Raman image processing, the Imaging Analysis software includes a ‘Registry’ of possible functional groups or other relevant compound information based on peak height or area calculations. After peak height or area calculation is developed, it can be saved to the Registry for future analysis use. The registry includes the peak calculation information and a ‘label’ describing the relevant vibrational motion. Image maps can be developed from mapping data simply by clicking on the desired, registered calculation to obtain a false color image of the peak intensity data for the registered calculation.
